Jornal de Química Experimental de Alimentos

Formation of Volatiles in the Lipoxygenase Pathway as Affected by Fruit Type and Temperature

Abstract

Yafei Han,Sheryl Barringer*

Objective: The objective of this study is to study the effect of temperature, lipoxygenase activity, substrate and fruit type on formation of volatiles in the LOX pathway. Methods: Volatiles in the headspace of tomato, bell pepper, tomatillo, strawberry, apple and pear were measured during and after fruit tissue disruption in real time using selected ion flow tube mass spectrometry (SIFT-MS) at 4, 25, 37 and 45°C for 60 min, and were compared to lipoxygenase (LOX) activity and fatty acid concentration. Results: In tomatoes, bell peppers and tomatillos, (Z)a-3-hexenal, (E)-2-hexenal and hexanal increased rapidly after blending, peaked and declined, while in strawberry, apple and pear they sometimes peaked and sometimes remained constant over the 60 min. In tomato and bell pepper, the peak levels of (Z)-3-hexenal, (E)-2-hexenal and hexanal in the LOX pathway had good correlations to LOX activity, which was greatest at 25°C. In contrast, tomatillo, strawberry, apple and pear had lower LOX activity, their 6C aldehydes did not show strong correlation with LOX activity, and the volatiles increased with increasing temperature to 45°C. The concentrations of hexen-1-ol and 1- hexanol did not correlate to LOX activity for any of the fruits. In tomato, bell pepper and tomatillo, there was no significant correlation between the ratio of linoleic acid:linolenic and the ratio of hexanal:hexenal. In strawberry, apple and pear, there were good correlations between linolenic acid content and concentrations of (E)-2-hexenal, hexenal and hexen-1-ol. Conclusion: Thus the effect of enzyme activity and substrate composition on formation of volatiles in the lipoxygenase pathway remains complicated and difficult to predict.