Jornal de Microbiologia Alimentar e Industrial

Serological, Bacteriological, and Molecular Investigation of Brucellosis in Bovine in Four Egyptian Governorates

Abstract

Nour Hamid

For testing purposes 347 documented positive and negative serum samples were collected from large ruminants with a history of Brucella melitensis infection. The buffer acidified plate agglutination test (BAPA) achieved highest relative sensitivity. In the case of BAPA, Rose Bengal Plate (RBPT), indirect ELISA (iELISA) and rivanol (Riv. T) tests, the kappa (π) agreement assessed for both species indicated a substantial agreement (p possibly 0.05). The diagnostic performance of serological tests in cattle was arranged in descending order as follows, according to the data obtained from the receiver operating characteristic curves (ROCs), the area under the ROCs and the diagnostic odd ratio; BAPAT, Riv. T, RBPT, iELISA, EDTA-modified microagglutination test (EDTA-mMAT), and MAT. In buffaloes the equivalent picture was, Riv. T, RBPT, BAPAT, iELISA, EDTA-mMAT and MAT.