Jornal de Biomarcadores Moleculares e Diagnóstico

Analysis of Total Urine Proteins: Towards a Non-Invasive Approach for Diagnosis of Visceral Leishmaniasis

Abstract

Om Prakash Singh and Shyam Sundar

Diagnosis of Visceral Leishmaniasis (VL) is a major obstacle in the control of this disease, as demonstration of parasite by splenic or bone-marrow aspiration is almost impossible in the peripheral rural areas because of lack of facilities. In order to identify a non-invasive biomarker for VL, we employed two-dimensional electrophoresis (2D-PAGE) coupled with mass spectrometry through a comparison of urinary proteome. Though, we did not find the biomarker protein for VL, our findings provide some basic insights for future development of non invasive diagnostic tool.