Jornal de Genética e Pesquisa de DNA

Screen of RNA aptamers to target CHS5 chitin trafficking signal as anti-fungal

Abstract

Vahed Mohammad

Chitin is a glycan composed of 1, 4-linked N-acetyl-D-glucosamine that exist in cell wall of Saccharomyces cerevisiae (S. cerevisiae). One of the emerging fungal pathogens is S. cerevisiae, when host defenses are weakened. S. cerevisiae strains show a specific transcription pattern after human blood infection and sequencing of genomes in yeast and mammals that encode very similar proteins. They make systemic infection and even death in the worst scenarios. In this work, in silico approach to screening RNA with potential binding affinity to a desired Chs5 (Chitin localization protein agent) to block chitin transferred. Atom coordinate of Chs5 were extracted from one of the conformations which had been determined by solution nuclear magnetic resonance (NMR) spectroscopy (PDB accession code: 4WJW).