Genética Humana e Embriologia

FSHR Thr307Ala Polymorphism Associates with Ovarian Response to Exogenous FSH Stimulation, However, Asn680Ser Seems Controversial

Abstract

Roghayeh Asl Monadi, Hourieh Khani , Parisa Mousapour, Masoud Maleki *

Objective: The success rate in assisted reproductive technologies is dependent upon the number of retrieved oocytes and available embryos to transfer. Administration of exogenous FSH is helpful reaching the optimum effectiveness; however, the ovarian responses to such stimulations are unpredictable among individuals. Two SNPs within FSH receptor gene can alter these responses and can be used for predicting the results. In the present study, rs6165 and rs6166 polymorphisms of FSHR gene were analyzed. Materials and Methods: In this case-control study 30 women with Poor Ovarian Response (POR), 55 women with Normal Ovarian Response (NOR) and 55 control women were analyzed. Target polymorphisms were detected using PCR-FRLP method. Bsu36I and BsrI restriction enzymes were used for detection of rs6165 and rs6166 SNPs, respectively. Results: The frequencies of A and G alleles for Thr307Ala (rs6165) polymorphism for control, POR, and NOR groups were 57.3%, 42.7%, 41.7%, 58.3%, 47.3%, and 52.8%, respectively. A and G allelic frequencies for Asn680Ser (rs6166) polymorphism in control, POR, and NOR groups were 63.6%, 36.4%, 53.3%, 46.7%, 51.8%, and 48.2%, respectively. Rs6165 SNP was more frequent among POR group comparing to control and NOR groups. Regarding rs61666 polymorphism, it was revealed that the differences of genotype frequencies between the analyzed groups were not statistically significant. Conclusion: Our results indicate the association of FSHR Thr307Ala polymorphism with the ovarian response to exogenous FSH stimulation; however, statistically significant differences between poor ovarian response infertile women and control group as well as good ovarian response group regarding the second polymorphism was not observed.