Revista de Bioprocessamento e Biotécnicas

Recovery of β-Amylase by Hybrid UF Membranes from the Soy Whey Wastewater

Abstract

Liming Zhao, Chaoqin Chen, Yongjun Qiu, Jiachun Zhou, Lihua Jiang and Yue Hugh Guan

Large-scale manufacturing of soybean proteins results in huge volume of “wastewater” which contains substantial amount of β-amylase, and the recovery of β-amylase has potential high value. In order to evaluate the feasibility and effect of novel hybrid Ultrafiltration (UF) method against the normal method, the pretreated effluent was concentrated by a low molecular weight cut off (MWCO) 3 kDa UF membrane firstly, terminated at Volume Concentration Ratio (VCR) of ca.7. To this UF retentate was then precipitated by adding 1.2M (NH₄)₂SO₄ to remove other proteins. The supernatant obtained following centrifugation was purified through a further higher 30 kDa MWCO UF membrane, which ended at VCR of ca.10. The prepared was purified 4.8 fold, with an enzyme activity concentration ratio of 61, and is associated with 74% combined recovery for the enzyme activity, giving a final specific activity of 14 U/mg (674 U/mL), of which enzyme activity is fully enough for the industrial applications. Preliminary data of this research indicate that this could be an effective method for treating kinds of related protein wastewaters.