Revista de Bioprocessamento e Biotécnicas

Properties of the Entomoparasitic Nematodes (Heterorhabditis bacteriophora) Liquid Culture using a Helicoidal Ribbon Agitator as Rheometric System

Abstract

Diola-Marina Nuñez-Ramirez, Luis Medina-Torres, Fausto Calderas and Guadalupe Sanchez-Olivares

The rheological parameters: flow behaviour index n, flow consistency index K and effective viscosity ηe were estimated for the entomoparasitic nematodes Heterorhabditis bacteriophora liquid broth at different culture maturation times. The nematode or nematodes were cultivated during 20 days in a bioreactor, and the growth media inside the bioreactor was enriched with protein and fat sources. Rheological parameters for the heterogeneous suspension were estimated with mixer principles employing a helical ribbon agitator fixed to a rheometer. As the culture matured, n decreased from 0.8 to 0.2 (-) and K increased up to 1200 mPa•sn; ηe showed a non-Newtonian (n<1) behaviour, ηe reached peak values of 0.32 Pa•s for a rotational speed of 0.5 revolutions per second (rps) and 0.048 Pa•s for 2.5 rps. Rheological properties reported here could be more reliable as compared to those reported for non-homogeneous liquid fermentations where estimations were performed with conventional geometries (i.e. concentric cylinders) which are appropriate for homogeneous systems but not for non-homogeneous ones.