Revista de Bioprocessamento e Biotécnicas

Effect of Inducers and Physical Parameters on the Production Of L-Asparaginase Using Aspergillus Terreus

Abstract

Moses Jeyakumar Rajesh, Leelavathy Rajesh, V.Satya veni, G.Thirumurugan, O.S.Sethuraman, Rajaram Sivasubramanian

An effort has been made to find the best medium and operating conditions for the production of extracellular L-asparaginase by Aspergillus terreus in shake culture fermentation through stepwise optimization strategies like studies on the effect of pH and temperature on L-asparaginase, studies on the enzyme activity in various concentration of L-glutamine and study the growth kinetics for L-glutamine medium. Our study shows that L- glutamine combined with L-asparagine influence the asparaginase production level and physical parameter such as pH and temperature has more contribution in enzyme production. The pH 8 and temperature 35°C found to be good environmental condition the production of extracellular L-asparaginase by Aspergillus terreus.