Tecnologias e pesquisa de transplante

A Novel Technique for Harvesting Hepatic Vein Blood and Time Course of Posthepatic Reactive Oxygen Species after Liver Transplantation in Rats

Abstract

Changku Jia, Youke Chen and Honglei Liu

Objective: To introduce a method for harvesting pure hepatic vein (HV) blood and to determine time course and peak level of posthepatic (HV blood) reactive oxygen species (ROS) after liver transplantation. Materials and methods: Blood was taken from HV and infrahepatic vena cava (IH-VC) for malondialdehyde (MDA) detection at different time points after transplantation.

Results: Pure posthepatic blood from HV was successfully obtained using the technique of IH-VC cannulation. This method guaranteed the harvesting of pure posthepatic blood from HV and pure peripheral blood from IH-VC. MDA concentration in HV elevated obviously with time and peaked at 1h after transplantation. MDA concentration at 1h was significantly higher than that at other time points except for 5 min. Furthermore, MDA concentrations in HV were significantly higher than that in IH-VC at 20 min, 30 min, 1h ( P <0.05) and slightly higher at all other time points. MDA concentration within IH-VC peaked at 2h after transplantation.

Conclusion: We introduced a novel technique for harvesting pure posthepatic blood from HV using the manipulation of IH-VC cannulation. We provided the first evidence that posthepatic ROS concentration elevated with time and peaked at 1h after transplantation.