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Volume 10, Emitir 3 (2021)

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Production of Recombinant Proteins from the Secretions of Lucilia Sericata Larvae for Wound Healing

Masoumeh Bagheri

Maggot therapy is a useful, effective and controlled method using  sterilized larvae Lucilia sericata to treat a variety of acute and chronic wounds. Larval secretions include digestive enzymes, antimicrobial peptides, growth factors, and wound healing agents. However, the use of maggot therapy is an effective method of wound healing.  But, this method has limitations such as disgust and fear of people (placing insect larvae on the wound), long duration of treatment, sterile breeding of larvae, etc. Therefore, using biological science and genetic engineering, it is possible to identify and produce recombinant proteins in larval secretions and then use them in wound healing. Very little research has been done on the production of recombinant proteins in Lucilia sericata larvae. The production of recombinant proteins in Lucilia sericata larvae could revolutionize the pharmaceutical and biotherapy industry and reduce wound healing costs

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A Novel Variation in the BSCL-2 Gene in Congenital Lipodystrophy

R. Turner, J. Polanco, M. Pietruszka, S. Tayefeh, Y. Valles-Ayoub, and M. Robinson

Lipodystrophy is a disorder of fat distribution and storage in the body and results in a generalized or regional selective loss of subcutaneous fat [1]; it may be acquired or congenital. Various syndromes have been described based on age of onset, body fat loss distribution, and associated comorbidities [1]. Attempts have been made to further characterize differences based on genetic testing. We describe a 33-year-old female patient who was referred for endocrine evaluation and in whom results from genetic testing of the BSCL gene showed 2 intronic homozygous variations that may be associated with congenital generalized lipodystrophy type 2 or, Berardinelli-Seip syndrome. This syndrome is a rare autosomal recessive disorderease characterized by a generalized lack of adipose tissue [1].  Results of genetic testing suggests a not previously described variation of type 2 lipodystrophy associated with atypical phenotypicalysical features and clinical course. The homozygous intronic variations present on intron 5 is NM_001122955.3 c.765+69A>G rs2850596 and the novel variant (c.766-49 T>C).

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Housefly (Musca domestica): A possible Mechanical Vector in the Transmission of Coronavirus Disease

Masoumeh Bagheri, Leila Izadpanah

Housefly is one of the most important health pests in the world. This species can mechanically transmit a large number of bacterial, viral, and parasitic pathogens. Coronavirus is one of the largest and most important  family of viruses among humans and animals. Coronavirus disease 2019 (COVID-19), the new Coronavirus, which can cause mild, moderate, severe symptoms and death in patients. Unfortunately, Coronavirus disease is spreading rapidly in the world, And there is no comprehensive information on the routes of transmission of this disease. Mechanical transmission through insects, can be one of the possible methods of transmission of the coronavirus, Houseflies can mechanically transmit coronavirus between humans and animals. There is no definitive cure for COVID-19, although vaccination is the best way to control this disease, but until the vaccine is fully and effectively discovered, the most effective method is to eliminate and reduce the routes of transmission of coronavirus disease. Insect removal and control methods (environmental improvement, mechanical and chemical methods can be one of the effective methods in interrupting the epidemiological cycle of coronavirus transmission.

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In Vitro Culture of Date Palm (Phoeruix dactylifera L) Roots

Fatima Musbah Abbas

The accessional harvested from the 10-year-old Wad Loggi, date palm (Phoeruix dactylifera L) class trees planted in open field conditions. Several treatments were performed with the aim of this work to obtain plant roots free of fungal or bacterial contaminants. The root is repeatedly washed with running tap water followed by soaking for different exposure times at different concentrations of “Clorox”. The first experiment results showed that for 60 min soaking with a concentration of 30% “Clorox” gave a relatively higher number of contaminant-free culture tubes. In the second experiment, explants were immersed in an antioxidant solution as a pre-treatment for the “Clorox” for different exposure times. Presoaking in this solution for 60 min led to significant increases in the percentage of sterile implants compared to the “Clorox” treatment alone. A comparative study was conducted using utilizing antiseptic iodine as potential chemical sterility for date palm root explants and as an alternative to “Clorox” In the third experiment, the roots were soaked for different treatment duration in an iodine solution. The results of iodine treatment gave consistently higher rates of sterile root explants compared to “Clorox” treated roots yet were associated with paired iodine treatment at all times of exposure. Thus, the most advisable sequence of disinfestations for date palm roots obtained from mature trees appear to be as follows: frequent washing repeatedly with running tap water before immersing in an antioxidant solution for 60min. The surface of the soak-treated roots in “Clorox” solution for 60 min, rinsed three times before implanted in a nutrient medium.

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Cell Membrane Structure and Its Properties

Abdul Razzaq

Cells are secured by a particular layer and, for bacteria, fungi, and algae, by a solid cell wall. Nonetheless, a few results of present-day biotechnology are created intracellularly and must be discharged to be thought and further decontaminated. This might be accomplished by cell penetration or lysis, which is known as cell disturbance.

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