Jornal de Histologia Molecular e Fisiologia Médica

Tissue microarray (TMA) is a new technology that has been created to improve the effectiveness of molecular profiling in cancer research by allowing researchers to quickly conduct large-scale investigations while lowering experimental variables and preserving valuable tissue samples. The approach enables the simultaneous examination of protein or genes in an array of hundreds of paraffin-embedded cored tissue specimens on a single glass slide. Immunohistochemistry, fluorescent-dye tests, and in situ hybridization can all be used to assess TMAs (ISH). TMAs enables the quick analysis of normal and cancer tissues and are particularly valuable for cancer biomarker validation investigations.

Pathology focuses on the morphological aspects of disease research for the most part. Molecular techniques are used in conjunction with pathology to isolate and identify specific infectious disease agents, to give more accurate illness diagnosis, to define the function of differential gene expression in disease aetiology, and to provide customised medicine approaches to therapy. In both clinical and anatomical pathology laboratories, molecular approaches have become indispensable. Many molecular applications in anatomical pathology have demonstrated their worth and effectiveness. Molecular approaches can help define disease more precisely, discover predictive and prognostic markers, and aid in the identification of novel molecular targets for specific therapeutic techniques.

In the presence of divalent metal ions (Mg2+, Mn2+, Co2+, and Zn2+), fructose 1,6-bisphosphatase catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Gomori was the first to discover the enzyme (1943). Approximately 2000 articles on the kinetics and tissue distribution of FBPase were published during the next 60 years. Only a few of these studies, however, deal with the enzyme's subcellular distribution. FBPase was discovered in the perinuclear area of hepatic and renal cells by Saez et al. in 1996, and nuclear localization of FBPase in these cells was discovered by Yanez et al. in 2003.

Esophageal cancer (EC) is a very common malignancy; 50% of cases had distant metastases at the time of diagnosis, and the majority of patients are men. Male EC (MEC) and female EC (FEC) clinicopathological features and metastatic patterns were compared in this study (FEC). Furthermore, risk variables linked to MEC prognosis were examined. The population for this study was taken from the Surveillance Epidemiology and End Results database. The descriptive analysis, the Kaplan-Meier technique, and the Cox regression model were used to assess MEC features and variables related to prognosis. 

Background: This case report emphasizes the recent studies in small bowel Endometriosis i.e. Endometriosis is an often painful disorder in which tissue similar to the tissue that normally lines the inside of the uterus- the endometrium grows outside the uterus.

Case presentation: A 41-year-old woman went to the emergency room with abdominal pain as her main complaint. The patient had been suffering from persistent stomach pain for almost two months, with the pain in her right lower abdomen getting worse. The ED physician determined a clinical diagnosis of small bowel obstruction based on a physical examination, relevant laboratory results, and radiographic studies. The most important radiological examinations, an abdominal X-ray and a CT scan of her belly and pelvis, revealed brief segments of thickened small intestine wall and dilated bowel loops, which were compatible with a clinical diagnosis of small bowel obstruction.