Biologia Molecular: Acesso Aberto

The Effect of Citrullus Colocynthis on the Reduction of InflammatoryAgents in Osteoarthritis

Abstract

Mohadese Akhzari, Maryam Mirghiasi S, Mahnesa Vassaf, Maryam Sadat Mesbahi Bidgoli and Zahra Shirkhodae Tari

Nowdays, the use of medicinal plants has been very common. Colocynth, Scientific name is Citrullus colocynthis in traditional medicine used to relieve pain and inflammation. The of aim of this study is to evaluate the effects of ethanol extract of Colocynth root on pro-inflammatory cytokine COX-2, INOS, IL-1β , TNF-α and NO, PGE2 on Inflammatory cells, similar osteoarthritis in chondrocyte cells and monocytes/macrophages and then treating them. At first the ethanol extract of the Citrullus colocynthis plant was prepared from the Iranian biological resource center. Then chondrocyte cells and THP-1 monocyte / macrophages (5 × 10⁵ cells/well) were incubated at a humidity of 90%, 5% CO₂, 37ºC for 72 h with control media alone or Citrullus colocynthis at concentrations of 70 μg/ml. One set of cells was activated by 20 μl LPS 20, then active cells are exposed to ethanol extract. And finally, cells tested with two control, cells and inflamed cells with LPS20, then results were evaluated Real Time PCR. Our review of the three levels of normal cells and inflamed cells and cells treated with ethanol extract of roots of Citrullus colocynthis plant with 20 ng/ml LPS20 showed that plant can reduce the expression of inflammatory cytokine and pro–inflammatory cytokine COX-2, INOS, TNF-α in Chondrocyte cells and reduced expression levels of TNF-α in THP-1 monocytes / macrophages and was reduced production of NO, PGE2. Our observations indicated that ethanol extract of root Citrullus colocynthis can reduce expression levels of pro-inflammatory cytokines in inflamed cells caused by situation same osteoarthritis, and we can use this plant for the treatment osteoarthritis in the future.