Biologia Molecular: Acesso Aberto

Synthesis of Silver Nanoparticles using Methanolic Extract of Salvia Officinalis L. and its Antibacterial Activity

Abstract

Rehab Omer Elnour*, Fatima Musbah Abbas Mohammed Elamin, Ghada M, Ahlam Salih Eltahir, Azhari Hamid Nour and Rwda Hassan Ahmed

Due to its numerous applications in numerous branches of technology, medicine and other study domains, nanotechnology is regarded as a highly developed technology. The main objectives of this work are to synthesize silver nanoparticles from silver nitrate using methanolic Salvia Officinalis L. aerial parts extract, to characterize the synthesized nanoparticles using Ultra Violet and Fourier Transform Infrared Spectroscopies and to test its antibacterial activity compared with standard antibiotics. The manufacture of silver nanoparticles was done using methanolic extract of Salvia officinalis L. (mramia). Two Gram negative bacteria E. coli and Pseudomonas aeruginosa and 2 Gram positive bacteria Bacillus subtilis and Staphylococcus aureus were used in this study. The creation of nanoparticles is indicated by a change in color from yellow to brown. The maximum absorption was found to be at 220 nm. The spectra of S. officinalis extracts displayed a broad and strong absorbance peaks at 995.20 cm^-1 that corresponds to Amines, 2929.67 aldehyde, 2360.17 carboxyl acid, 1691.46 aldehyde, 1456.46 is NO₂, 1382.87 ether and 1031.85 alcohol. The methanolic extract of the S. officinalis and the synthesized nanoparticles were tested against the studied bacteria, the results of the methanolic extracts were higher in its antibacterial activity than the activity of the synthesized silver nanoparticle and this explains the reason for using this plant in folk therapy.