Microbiologia Médica e Diagnóstico

Surgical Site infections: Distribution Studies of Sample, Outcome and Antimicrobial Susceptibility Testing

Abstract

Rama Bastola, Pramila Parajuli, Anjita Neupane, and Anjan Paudel

Background: The present study was conducted in the clinical lab of Microbiology department, College of Medical Sciences. The samples that were sent for culture and sensitivity from patients developing signs and symptoms collected from patients developing symptoms of SSIs in different wards as well as ICUs of this hospital were included in this study. A total of 206 pus samples were processed and result was interpreted on the basis of different tests. Isolation and identification of the organism was done by gram stains and culture growth. Antibiotic susceptibility test was performed by Kirby Bauer disc diffusion method and result was interpreted as per Clinical and Laboratory Standards Institute (CLSI) guidelines.

Result: Out of 206 clinically suspected cases 106 (51.4%) were culture negative and 100 (48.6%) were culture positive. The growths were found to be higher in male patients than in female patients. The most commonly isolated pathogens were Staphylococcus aureus 24 (21.8%) and Escherichia coli 24 (21.8%), followed by Pseudomonas aeruginosa 17 (15.4%). Amikacin (86.4%) was the most sensitive drug. 14 isolates of Methicillin resistant S. aureus (MRSA) were found.

Conclusion: The study helped in the assessment of the current anti-microbial resistance patterns of bacterial isolates in post-operative wound infections and helps in formulation of the strategy to reduce the infection rate in College of Medical Sciences-Teaching Hospital, Bharatpur.