Jornal de Citologia e Histologia

Role of Fat Feeding on the Diabetic Albino Rats

Abstract

Ahmed H. Abdel-Rahman H. El-Rashedy, Mohamed H. Mostafa Wahdan, Khaled Abdel-Fattah R. El-Sabban, Mohamed Ali Khadrawy, Tamer M. M. Abu-Amara, Hanan A.Al-Hamaky and Nahid M. El-Hagar

Aim of the work: The nutrition of developing countries are rapidly changed and associated with high incidence
of obesity as well as the metabolic syndrome and type 2diabetes. There is an increased intake of high caloric foods
rich in refined carbohydrates, fats and red animal proteins instead of low caloric foods rich in fibers and containing
low fats. This study aimed at the evaluation of effect of animal fats on the diabetic rats.
Material and methods: This study was performed on forty eight albino rats (their body weight was 190 ± 10
grams) divided equally into four groups. Two groups (III & IV) were given intravenous streptozotocin in a concentration
of 60 mg /Kg. B.W. for three days in order to induce diabetes through the destruction of pancreatic islet beta cells.
Non-diabetic rats (group I) were given the laboratory diet (LD) while the non-diabetic second group of rats received
an emulsion rich in fat. Third group diabetic animals were fed on the laboratory diet (LD) while group IV diabetic
rats were received high-fat (HF) emulsion in the same dosage as group II. C-peptide (ng/ml), blood glucose (mg/
dl), serum triglycerides (TG; mmol/l) and total cholesterol (TC; mmol/l) were assessed before the beginning of this
experiment and also once every two weeks for four months in non-fasted diabetic and non diabetic counterparts.
Moreover, kidneys and liver were examined histopathologically to detect fatty intracellular accumulation and any
other changes.