Biologia Molecular: Acesso Aberto

Red Blood Cells Molecular Biology

Abstract

Sudhansu Sekhar Patra

We organized extremely oriented, multi-lamellar stacks of human red blood cell (RBC) membranes used on silicon wafers. RBC ghosts were organized by hemolysis and used onto functionalized silicon chips and annealed to the multi-lamellar RBC membranes. High steadfastness X-ray diffraction has been used to regulate the molecular structure for the stacked membranes. We current direct investigational suggestion that these RBC membranes encompass of nanometer sized fields of essential coiled-coil peptides, as well as liquid ordered (lo) and liquid disordered (ld) lipids. Lamellar spacings, membrane and hydration water coating depths, areas per lipid tail and domain sizes were resolute. The mutual drug aspirin was additional to the RBC membranes and originate to interrelate with RBC membranes and rather partition in the head group area of the lo domain principal to a fluidification of the membranes, i.e., a thinning of the bilayers and a growth in lipid tail spacing. Our consequences further provision present models of RBC membranes as patchy structures and provide unprecedented structural specifics of the molecular organization in the changed domains.