Guoyan Liu, Chunyan Chai and Bing Yao
In this study, anti-Salmonella polyclonal antibodies immobilized on cellulose nitrate membrane were used to capture Salmonella pullorum (S. pullorum) in biological samples. The rapid evaluation of S. pullorum contamination was based on the analysis of the activities of catalase, a biomarker of this bacterium. After a screen printed electrode (SPE) modified with multi-wall carbon nanotubes (MWCN)-chitosan-peroxidase was connected to a portable selfmade amperometric sensor, the determination of S. pullorum contamination was carried out by adding the reaction product, which was obtained from the hydrogen peroxide dismutation catalyzed by the bacterial catalase, to the reacting area of the SPEs. A working potential of 0.55 V was applied in the sensing system and the current value displayed on the amperometric sensor was used as the detection signal. This method allowed the quantification of S. pullorum with the detection limit of 100cfu mL-1 in culture media and chicken samples. The stability, reproducibility and sensitivity of the modified SPE were also investigated. Moreover, successive analysis was conveniently accomplished by replacing the one-off SPE. This portable sensing system is a rapid, cost-effective and straightforward approach for screening S. pullorum contamination in food samples.
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