Journal of Bioengineering & Biomedical Science

Physicochemical and Biological Characterization of Nanofibrous ε-Polycaprolactone Matrices with Nano-hydroxyapatite and Humulus lupulus L. Extract for Oral Applications

Abstract

Jeanne Amy

This study presents the physicochemical and biological characterization of nanofibrous ε-polycaprolactone (PCL) matrices incorporated with nano- Hydroxyapatite (nHA) and Humulus lupulus L. (hops) extract for potential oral applications. The electrospun Nanofibrous matrices were fabricated using a blend of ε-polycaprolactone polymer, nano-hydroxyapatite, and hops extract. Physicochemical analyses including Scanning Electron Microscopy (SEM), Fourier-Transform Infrared Spectroscopy (FTIR), and X-Ray Diffraction (XRD) were performed to assess the morphology, chemical composition, and crystallinity of the matrices. Biological characterization involved in vitro evaluation of cytocompatibility using Human Gingival Fibroblasts (HGFs) and antimicrobial activity against oral pathogens. The results demonstrate the successful fabrication of nanofibrous matrices with well-defined morphology, enhanced mechanical properties, and controlled release of bioactive compounds. Moreover, the matrices exhibit excellent cytocompatibility and antimicrobial efficacy, suggesting their potential for various oral applications including periodontal tissue engineering and drug delivery systems.