Revista de Bioanálise e Biomedicina

Pharmacokinetic Evaluation of Metolazone Tablets using healthy Human Volunteers

Abstract

Basvan Babu, Selvadurai Muralidharan, Subramaniya Nainar Meyyanathan and Bhojraj Suresh

A sensitive and reproducible high performance liqui d chromatography (HPLC) method has been developed and validated for the quantification of metolazone in h uman plasma, after solid phase extraction (SPE). A Good reso- lution was achieved on a reverse-phase LichroCART Purospher ® C 18 column using the mobile phase acetoni- trile–0.5% triethylamine (35:65) in isocratic eluti on with a total run time of 15 min. The analyte, metolazone , was detected by using high performance liquid chromatog ra- phy with the support of photo diode array detector. Limit of detection and Lower limit of quantification was found to be 1 and 2.5 ng/mL. The present method was succe ss- fully applied in the pharmacokinetic study of metol azone in human plasma.