Biologia Molecular: Acesso Aberto

Molecular Detection of JCV in Urine and Blood Samples of Renal Transplant Patients in Khartoum State, Sudan

Abstract

Badri AM, Janay A, Jonas H, Liban AH, El Fadol ASM, El Hussein ARM, Elkhidir IM and Enan KA

Background: The present study aimed to investigate the infections of Polyomaviruses JCV (John Cunningham virus) in renal transplant recipients in Khartoum State in Sudan and to compare the results of the detection of JCV in urine and plasma.

Methodology: A total of 50 Plasma and 50 urine samples were collected randomly from renal transplant recipients attending Sudanese Renal Transplant society during period between January and February 2016. The samples were subjected to polymerase chain reaction (PCR) assay to detect the viral deoxyribonucleic acid (DNA).

Results: Among the 50 renal transplant patients the Polyomaviruses nucleic acid was detected in 24 plasma specimens (48%) and only in 14 urine specimens (28%) by using conventional PCR method.

Conclusion: JC Virus is related to clinical manifestations among renal transplant patients. The routine use of PCR on urine and plasma is a useful tool for the rapid and sensitive detection of JC virus in renal transplant recipient.