Biologia Molecular: Acesso Aberto

Identification of DJ-1-Associated Regions on Human Genes from SH-SY5Y Cells using Chromatin Immunoprecipitation Sequence Technique

Abstract

Takuya Yamane, Naoyuki Sugimoto, Hiroshi Maita, Kazufumi Watanabe, Kazuko Takahashi-Niki, Chinatsu Maita, Izumi Kato-Ose, Shizuma Ishikawa, Jian-wei Gao, Hirotake Kitaura, Takeshi Niki, Sanae MM Iguchi-Ariga and Hiroyoshi Ariga

DJ-1, a cancer- and Parkinson’s disease-associated protein, works as a coactivator to various transcription factors. In this study, DNA fragments that bind to DJ-1 complexes were obtained by a chromatin immunoprecipitation sequencing with an anti-human DJ-1 antibody using chromatin from SH-SY5Y cells. We identified 60 different sequences as potential DJ-1 complex-binding sites in genes. Of sequences identified, expression levels of DJ-1-associated sitecontaining genes for DNA polymerase N, estrogen receptor α and S-adenosylhomocysteine hydrolase like-2 were decreased in DJ-1-knockdown cells and in 6-OHDA-treated cells. These studies suggest that DJ-1 regulates the expression of versatile genes at the transcriptional level and that some of the genes are regulated by DJ-1 in an oxidative status-dependent manner.