Biossensores e Bioeletrônica

Glucose Oxidase Modified Electrodes of Polyaniline and Poly (aniline-co- 2-anilinoethanol) as a Biosensor: A Comparative Study

Abstract

Ali Keyhanpour, Seyed Mohammad Seyed Mohaghegh and Ahmad Jamshidi

A simple technique is described for constructing a glucose sensor so that enzymes, glucose oxidase (GOD) was immobilized by cross-linking via glutaraldehyde 0.1% at 0.1 M phosphate buffer with 7.0 pH in a polyaniline and poly(aniline-co-2anilinoethanol) thin films, which were electrochemically deposited on a platinum plate in phosphate and acetate buffer. The results of EIS and SEM indicated the successful immobilization for enzymes in the polymers film. The maximum current response was observed for the electrodes at pH 7 and potential 0.65 V (versus Ag/ AgCl). The poly (aniline-co-2anilinoethanol)/GOD electrode gives high stability and fast response as compared to polyaniline/GOD electrode in amperometric measurements so results show that the poly (aniline-co 2anilinoethanol) electrode is more suitable for biological systems.