Revista de Bioanálise e Biomedicina

Estimation of Swertiamarin in Enicostemma Littorale and Marketed Formulations Using HPLC-UV Method

Abstract

Prawez Alam, Mohammad Ali, Rahul Singh and Faiyaz Shakeel

A simple, economic, reproducible, robust and precis e HPLC method for estimation of swertiamarin in both 60 % methanolic extract of Enicostemma littorale and mar- keted formulations was developed and validated in p resent investigation. The mobile phase composed of methano l and water (90:10 % v/v), gave a sharp and well-defi ned peak of swertiamarin at the retention time of 10.15 ± 1.52 min. The limit of detection (LOD) and limit of quan tifica- tion (LOQ) were 17.25 and 56.92 μ g/ml respectively. The proposed method with high degree of precision and a ccu- racy was employed for the estimation of swertiamari n in methanolic extract and in marketed formulation.