Jornal de Citologia e Histologia

Effect of Triclosan on the Renal Cortex of Adult Male Albino Rats and the Possible Protective Role of Ellagic Acid: Histological and Biochemical Study

Abstract

Zeinab A. Hassan, Manal R. Abd El-Haleem and Ghada N. Mansour

Background: Triclosan (TCS) is widely used broad spectrum bactericide. Ellagic acid (EA) has radical scavenging properties.
Aim of the work: to assess the structure of the renal cortex after TCS administration and to determine the possible protective role of EA.
Materials and Methods: Thirty adult male albino rats were divided into three groups; control group, TCS treated group: TCS was administered 200 mg/kg, once daily for six week, by oral gavages. TCS-EA treated group: received in the same doses of TCS and EA (30 mg/kg) for six weeks. Blood urea nitrogen (BUN), serum creatinine (Sc) and uric acids (UA) were measured. Renal cortex samples were processed for light and electron microscope examination.
Results: Examination of TCS treated group revealed increased glomerular cellularity in some corpuscles. Podocytes showed effacement of the foot processes and focal thickening of the glomerular basement membrane. Some tubules showed marked cellular disorganization. Disoriented basal mitochondria, areas of rarified cytoplasm and electron-dense bodies were noticed. Intense positive caspase-3 reaction was expressed in the tubular cells. TCS-EA showed improvement of morphological organization of renal cortex glomeruli, proximal and distal tubules with moderately expressed caspase-3 in renal tubules Statistical analysis showed significant increase of BUN, SC and BUN in TCS treated group in comparison with the control group.Their levels in TCS-EA treated group showed a significant decrease in comparison with TCS treated group
Conclusion: In conclusion, TCS leads to alterations in the histological structure and functions of renal cortex of albino rats and EA supplementation could protect from these changes.