Microbiologia Médica e Diagnóstico

Differential Systemic MyD88-Dependent vs. MyD88-Independent Cytokine Ratio by Etiologic Agent in Adults with Severe Community- Acquired Pneumonia

Abstract

Purpose: Classifying bacteria early, before cultures results are available, would improve the choice of initial antibiotics in patients with severe pneumonia. TLR2 and TLR4 transduce signal through the MyD88-dependent pathway to stimulate IL-8 and TNF-α production. TLR4 can also signal through a MyD88-independent pathway to stimulate RANTES and IFN-β production. As gram-negative bacteria primarily activate TLR4, while gram-positive bacteria also activate TLR2, differential cytokine expression would be expected depending on specific bacterial etiologies.

Methods: Admission serum samples from 53 patients admitted to Medical Intensive Care Unit at the University of Maryland Medical Center between January 2006 and September 2013 were assayed for IL-8, RANTES, TNF-α, and IFN-β levels using the University of Maryland cytokine core laboratory and commercial ELISA kits. Cytokine levels and the ratio of MyD88-independent to MyD88-dependent cytokines, ([IFN-β] X [RANTES])/([IL8] X [TNF-α]) were compared to the culture identified organisms.

Results: 14 gram-negative and 17 gram-positive pneumonias were identified. None of the individual cytokine demonstrated statistically significant differences between the gram-negative and gram-positive infections. The ratio of MyD88-independent/MyD88-dependent cytokines was 111.1 ± 34.9 in gram-negative infections, 29.9 ± 8.3 in gram-positive infections, with p=0.04.

Conclusions: Serum MyD88-independent to MyD88-independent cytokine ratios significantly discriminated gram-negative from gram-positive pneumonia. As technology improves our ability to generate panels of cytokines quickly from clinical specimens, a strategy of pooled cytokine ratios based on underlying pathophysiology, as was done in our study, could guide clinicians in critical early antibiotic choices.