Sampada Sontakke, Kishan K Khetan, Subhodeep Banerjee, Tanmoy Mondal, Jagamohan Chhatai, Manika Pal-Bhadra and Utpal Bhadra
The RNAi technology is a revolutionarized and powerful mechanism for in built alternate cell defence of an organism. The foreign DNA intruders form double stranded RNA intermediate during their propagation, which further cleaved in small antisense RNA by the action of dicing enzyme Dicer and further coupled with the complementary RNA for nucleation. Now a days lot more transgene were developed that involved frequently different silencing tricks, in extreme cases threshold induced silencing, DNA elimination etc. In few organisms the hall mark of silencing are involved in unpaired DNA silencing in somatic cells. The simple co-suppression events and double stranded mediated RNA degradation creates a new technology RNAI for a readymade knock down methods, However beyond limiting unfold silencing strategies of different transgenes in transcriptional silencing and RNAi technology has imported connection that are subject to answer in the review. We imagine new more powerful technology may generate for readymade activation or silencing based on the recently invented different types of transgene silencing.
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