Biossensores e Bioeletrônica

Biosensing Applications of Graphene Amination by Antibodies toward Grafting

Abstract

Maxim Basedina

The easy combination of biografted 2D subsidiaries supplemented by a nuanced comprehension of their properties are cornerstones for headways in biosensing advances. The potential of aminated graphene as a platform for the covalent conjugation of monoclonal antibodies to human IgG immunoglobulins is thoroughly investigated in this paper. We investigate the chemistry and its effect on the electronic structure of the aminated graphene prior to and following the immobilization of monoclonal antibodies by utilizing core-level spectroscopy techniques, specifically X-ray photoelectron and absorption spectroscopies. Electron microscopy techniques are also used to examine how the derivatization protocols affect the morphology of the graphene layers. These findings advance and outline the application of graphene derivatives in biosensing as well as hint at the features of the alterations of graphene morphology and physics upon its functionalization and further covalent grafting by biomolecules. Chemiresistive biosensors are fabricated and tested, demonstrating a selective response towards IgM immunoglobulins with a limit of detection as low as 10 pg/mL.