Jornal de Citologia e Histologia

Antihyperglycemic Activity, Antihyperlipedemic Activity, Hepatoprotective Activity and Histopathological Analysis of Natural Honey in Streptozotocin Induced Diabetic Rats

Abstract

Asaduzzaman MD, Nahar L, Hasan M, Khatun A, Shajedul Haque M, Hasan N, Tamannaa Z, Huda N, Fazley Rabbi M, Ray M, Nur Islam M, Maniruzzaman M, Mobassirul Islam M, Dastagir N and Sarker S

Honey contains a variety of phytochemicals and other substances, such as organic acids, vitamins and enzymes, which may serve as a source for dietary antioxidants. Antioxidants shows improve insulin level and protect insulin resistant in diabetes mellitus. Honey bee venom reduces blood glucose level as well as lipid profile through increased insulin secretion and glucose take-up. For that reason, honey could be considered as a potential remedy for diabetes as well as Cardiovascular Disease (CVD). The objective of this study was to determine the medicinal activity of honey and its role on antihyperglycemic, antihyperlipidemic, hepatoprotechtive activity and histopathological analysis of natural honey in Streptozotocin- induced diabetic rats. The experimental rats were divided into six groups (n = 6). Diabetes Mellitus (DM) was induced by single intraperitoneal injection (65 mg/kg BW) of freshly prepared Streptozotocin hydrate solution in 0.9% saline solution. Hyperlipidemic was induced by mixture of cholesterol (1.5%) and cholic acid (0.5%) with diet of rats. At the end of treatment, the blood glucose level and lipid profile was measured by using commercial kits. Histopathology of liver and heart were performed of observed any changes in the cellular structures (degradation and regeneration) of the rats after receiving the sample for 28 consecutive days with respect to control group. Honey bee-treatment significantly decrease blood glucose level in diabetic rats. TC, TG, LDL, VLDL are significantly (p < 0.05) decrease whereas HDL significantly increase (p < 0.001). The SGPT and SGOT were significantly decrease (p < 0.05) which showed hepatoprotective activity of honey. Honey has favorable effect on the histopathological changes in Streptozotocin- induced diabetic rats. On the basis of above findings, it can be concluded that, supplementation of honey could significantly contribute to control blood glucose level as well as lipid profile in diabetic subjects. Honey showed prevent various complications of diabetes and improve some haematological parameters.