Toxicologia Ambiental e Analítica

The Growth Behavior of Chlorella vulgaris in Bisphenol a under different Cultural Conditions

Abstract

Lu Wang, Xiurong Chen, Hualin Wang, Yan Xu2 and Youjun Zhuang

The effects of different initial concentrations of bisphenol A (BPA) on Chlorella vulgaris and removal capacity of BPA by Chlorella vulgaris were investigated under the light and the dark cultural conditions. Experiments were performed in 250 mL flasks under light and dark conditions with different BPA concentrations. Results showed that 0-20 mg·L-1 BPA concentration under the light condition and 0-10 mg·L-1 BPA concentration under dark condition plays a promoting role on the growth of Chlorella vulgaris in terms of cell density. The effect of BPA removal under light condition was obviously better than that under the dark condition. The maximum BPA removal rates were 3.425 ± 0.145 mg (L·d)-1 and 1.530 ± 0.025 mg (L·d)-1 under two conditions and were observed during 2-4 d and 0-2 d, respectively. The largest removal amounts of BPA under two conditions were all investigated in L-BPA50 and D-BPA50 groups. Both superoxide dismutase (SOD) and catalase (CAT) activities were promoted in all the treatments, which proved that C. vulgaris showed a positive response to the BPA stress condition. SOD activity showed sensitive and responsive to the new medium since it was promoted immediately on the incubation day. CAT activity was supposed to be more tightly controlled in response to BPA because its level was related to the BPA removal.