Ciência e Tecnologia Veterinária

Studies on Thermo stability of Newcastle Disease Virus (Local Isolate) for Preparation of Vaccine

Abstract

Chakraborty D, Guha C, Biswas U, Chatterjee A, Jana PS, Biswas S, Sharma L, Pradhan N, Bhagat S and Naha BC

The study was carried out to assess the thermostability of Newcastle disease virus (local isolate) which was isolated, characterised and provided by the Department of Veterinary Epidemiology and Preventive Medicine, West Bengal university of animal and Fishery Sciences. The thermostability procedure consist of three cycles which were carried out at 25°C for 36 days, 37°C for 29 days and 56°C for time period ranging between 5 minutes to 8 hours, respectively. The results showed that the infectivity titre of the virus was remained good at the end of the first thermal cycle i.e. the HA titre i.e., 28 and 108.5 EID50/ml, respectively. At the end of the second thermal cycle the infectivity titre of the virus was also in good level i.e. 27 and 108.50 EID50/ml, respectively. At last the infectivity titre of the virus decreased to 25 HA titre and 107.36 EID50/ml, respectively in 10 minutes of thermal exposure at 56°C and the HA titre and EID50 value became undetectable from 15 minutes and onward till the end of the third thermal cycle.