Jornal de Patogênese Microbiana

Molecular and Histopathological diagnosis of cutaneous leishmaniasis in Urmia, Iran in 2020: Report of Five Cases

Abstract

Mohammad Yousefzadeh

Cutaneous leishmaniasis(CL) is the most common form skin disease caused by a single-celled parasite that is transmitted by the bite of a phlebotomine sand fly. bloodshed transmitted from infected animals rodents, dogs or humans to healthy humans can cause contamination. Symptoms usually appear three months to a year after the bite. Leishmaniasis affects almost 12 million people worldwide and it is one of the diseases that special attention should be paid tocontrol and prevent it.

Methods: In the laboratory, the wounds are prepared from the patients and from the interstitial fluid of the wounds using a smear scalpel blade. Also, for further examination and more definitive diagnosis of the patients' samples, biopsy was performed. In addition, serous fluid from the patient’s skin

lesion were cultured in Schneider’s Drosophila medium (Sigma, Germany), supplemented with 5% heat-inactivated fetal bovine serum (Sigma, Germany). The real time PCR method was performed using clinical samples and it was repeated twice for each batch of samples.