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Importance of Sampling Sites for Postmortem Evaluation of Ethyl Alcohol

Abstract

Ismail Özgür Can, Erdem Özkara, Serpil Salaçin and Mukaddes Gümüstekin

Detection of ethyl alcohol and its origin in postmortem specimens is essential in terms of medico-legal aspects. It is a complicated process to determine whether alcohol has been taken in the ante-mortem period and/or originates from postmortem endogenous production.

In this study, considering sampling sites and storage conditions, we aimed to develop an approach for postmortem ethyl alcohol investigations. Samples were collected from 32 cases. Blood specimens drained from the femoral vein and the vena cava inferior were put into well covered PET tubes with anti-coagulants and urine samples were put into well covered PET tubes without anti-coagulants and preserved at + 4ºC, analyzed with enzymatic immunoassay within five days of specimen collection.

Scene investigations, sampling and sampling sites, specimen handling, preserving specimens and preparations before analyses are highly important for an accurate and scientific evaluation of postmortem ethyl alcohol. There were significant differences in ethyl alcohol concentrations between blood from the femoral vein and vena cava inferior and urine. Femoral vein and urine specimens seemed to be more reliable than vena cava inferior specimens.