Ciência e Tecnologia Veterinária

Exploration of Viability of Ketorolac on Isolated Leydig Cells of Bucks (Capra hircus) In Vitro: Part I

Abstract

Mohanad A Al-Bayati* and Zena ZA AI-Luhapy

The present study was conducted to clarify the effect of ketorolac (NSAID) on Leydig cells of Buck to achieve the ketorolac event through two experiments. First is to determine the survival time of Leydig cells to produce maximum viable growth; The second is to assess the viability using multiple ketorolac series concentration of 0.03-0.15 in vitro. The viability of proliferative cell was decreased as dose dependent due to the negative correlation of the cessation, transition and development from the G2 phase to the intermediate M phase of cell cycle, which is the stopover proliferation of cells and diminishes the viability of isolated buck Leydig cells. So the inhibition of NF-κB through ketorolac may be augmenting the chemo-sensitivity of Leydig cells to the DNA topoisomerase inhibitor, which was presumably induction of apoptosis shown by DNA mismatch repair proficiency covered disruption of cell metabolism.