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Epithelial Cells Transfer: An Experimental Study

Abstract

Piters A, Esponda A, Monique G, Doutremepuich A, Fromentin S and Doutremepuich C

Objective: The objective of the present study was to analyze the conditions of epithelial cells transfer after an initial deposition on a first object to a second object (secondary transfer). This study should provide an experimental model which should contribute to better understand and analyze all the transfer mechanisms.
Methods: Using glass slides and cotton gauzes as supports for the transfer of epithelial cells from an object to another one, we analyzed the occurrence and efficiency of cells transfer in different conditions of hygrometry and contact.
Results: Our investigation reveals that after one hour deposition on a glass slide, the epithelial cells are no longer able to transfer to a second support. Moreover, our results also demonstrate that in opposition with the environmental factors such as hygrometry which are determinant for the transfer mechanisms, the strength of the contact between the glass slide and cotton gauze does not influence the cells transfer efficiency.
Conclusion: In conclusion, the secondary transfer of epithelial cells is only possible if the cells are not strongly attached to their support.