Vincenzo Cardella
Exploring the Therapeutic Potential of Stem Cell Cultured Conditioned Media: Insights from Zebrafish Embryo Evaluation Conditioned media obtained from stem cell cultures offer promising prospects as innovative therapeutic interventions against a range of diseases, owing to their rich reservoir of growth, trophic, and protective factors. Crucially, thorough in vivo assessment of these products' effects and safety is imperative. Zebrafish emerges as an ideal testing ground for high-throughput toxicological analysis, presenting an opportunity to minimize reliance on mammalian models while maintaining reliability. In this study, we delved into the biological ramifications of exposing zebrafish embryos to conditioned medium derived from Wharton's jelly mesenchymal stem cells. Employing a multifaceted approach involving molecular, embryological, behavioral, and in vivo imaging techniques, we unearthed a spectrum of outcomes arising from non-toxic/non-lethal dosages of the conditioned medium. Notably, this exposure triggered an array of responses including antioxidant fortification, anti-apoptotic activity, and pro-regenerative potential. This was underscored by the upregulation of several genes associated with antioxidant defense, glycolysis, and cell survival (bcl2l1, mcl1a, and bim). Simultaneously, the conditioned medium downregulated pro-apoptotic markers. Of note, this comprehensive investigation marks the pioneering attempt to thoroughly analyze the effects of conditioned medium on an entire organism, encompassing developmental, molecular, and behavioral perspectives. We hold a strong belief that these findings will lay a robust foundation for the future therapeutic utility of conditioned media.
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